It was the second consecutive game that Johnson has missed with the injury and comes on the heels of receiving a cortisone injection on Friday night to help deal with the lingering pain.
"He needs another day," Blue Jays manager John Farrell said. "He did receive a trigger-point injection to address the inflammation that's in that tendon behind the knee. So, he has soreness from the injection.
"The overall condition to the hamstring is roughly the same. In other words, he was able to play with it, he was managing the pain through some treatments, but we felt like it was getting to the point where the MRIs showed there was no structural issue but the inflammation was localized."
Johnson is listed as day to day, but Farrell said his second baseman would have preferred to play on Sunday. The club instead decided to take a cautious approach with the hope of getting rid of the inflammation so the discomfort does not linger for a long time.
The veteran is hitting .251 with eight home runs and 23 RBIs in 44 games and has been a mainstay at the top of the order.
"He feels like he could play, but we kind of took that decision out of his hands to give it 24 more hours to allow the treatment to take hold a little bit more and not to set him back at all," Farrell said.