The 24-year-old finished the night 1-for-2 with a double, and he was eventually replaced in the lineup by veteran outfielder Rajai Davis.
"He just tweaked something in his side," Blue Jays manager John Gibbons said. "He's had it before. I saw him grimacing a little bit. He should be alright tomorrow. We'll see. ... I noticed it on the swing, and I didn't want to take a chance."
Until Sierra returns, Davis will likely pick up the bulk of the workload in right field. That would keep rookie Kevin Pillar in the lineup at left, while Anthony Gose would continue to start in center.
Sierra is hitting .256 with seven extra-base hits and seven RBIs in 16 games this season. He spent most of the year playing for Triple-A Buffalo.
Gregor Chisholm is a reporter for MLB.com. Read his blog, North of the Border, and follow him on Twitter @gregorMLB. This story was not subject to the approval of Major League Baseball or its clubs.